Introduction to bulk RNA-Seq: From Quality Control to Pathway Analysis

Abstract

The Swiss Institution of Bioinformatics and the Chemistry | Biology | Pharmacy Information Center offer a two-day course on September 19–20, 2024, entitled Introduction to Bulk RNA-Seq: From Quality Control to Pathway Analysis. This course is designed for life scientists or bioinformaticians familiar with "Next Generation Sequencing" who wish to acquire the necessary skills to analyze RNA-seq gene expression data.

Learning Objectives

At the end of the course, the participants are expected to:    Describe the advantages and pitfalls of RNA sequencing    Design their own experiment    Perform the downstream analysis using command line software (QC, mapping, counting, differential expression analysis, pathway analysis, etc.)    Critically assess the quality of their results at each step of the downstream analysis

Content

This two-day course will present the theory and bioinformatics tools required to analyze RNA-seq gene expression data. The course will present the theory and bioinformatics tools required to analyze RNA-seq gene expression data, from the raw data to the biological interpretation. The following topics will be discussed:    Quality control    Mapping to genomes & transcriptomes    Read counting, gene & exons differential expression analysis    GO enrichment and pathway analysis

Location

• ETH Hönggerberg
  HCI

Target audience

Language